SCHISTOSOMIASIS RESOURCE CENTER

STANDARD OPERATING PROCEDURES

These Standard Operating Procedures are used in the Schistosomiasis Resource Center to maintain the life cycles of Schistosoma spp. The SOPs are divided into five categories.

Standard Operating Procedures

Image of S.mansoni schistosomula cultured in vitro

  1. Artificial pond water for the cultivation of Biomphalaria glabrata, Bulinus truncatus and Oncomelania hupensis
  2. Aquaria maintenance
  3. Growth of immature B. glabrata
  4. Snail egg clutch collection
  5. Growth conditions until patency
  6. Control of rotifers and other commensals living in snail aquaria
  7. Gel snail food preparation
  8. Preparation of mud base medium for growth of Nostoc (cyanobacteria)
  9. Fixing Biomphalaria glabrata snails for dissection

  1. Percutaneous exposure of mice to Schistosoma mansoni
  2. Perfusion of adult worms from infected mice
  3. Behavior of Schistosoma mansoni cercariae
  4. Preparation of in vitro schistosomules
  5. Collection of lung Schistosoma mansoni schistosomules
  6. Culturing schistosomula
  7. Isolating Schistosoma spp. eggs from murine liver and gut
  8. Microscopic evaluation of sporocyst development in live Biomphalaria glabrata
  9. Shedding cercariae from patent snails
  10. Exposure of snails to miracidia
  11. Positivity shedding
  12. Quantitating Schistosoma spp. cercariae

  1. Shedding Oncomelania for S.japonicum cercariae
  2. Collecting Schistosoma japonicum eggs from mouse tissues

  1. Maintenance of Bulinus truncatus truncatus
  2. Exposure of LVG Golden Syrian Hamsters to Schistosoma haematobium cercariae
  3. Perfusion of adult S. haematobium worms from infected hamsters
  4. Collection of S. haematobium eggs from hamsters

  1. Biomphalaria glabrata embryonic cell (Bge) culture

  1. Genomic DNA extraction from schistosome intermediate hosts and parasite stages
  2. Non-invasive DNA transfection to B. glabrata using polyethylenimine (PEI)
  3. Preparation of soluble egg antigen (SEA) S. japonicum eggs
  4. RNA extraction from schistosome intermediate hosts and parasite stages using RNAzol ®