These Standard Operating Procedures are used in the Schistosomiasis Resource Center to maintain the life cycles of Schistosoma spp. The SOPs are divided into six categories.
Procedures common to maintaining intermediate snail hosts of Schistosoma spp.
- Artificial pond water for the cultivation of Biomphalaria glabrata, Bulinus truncatus and Oncomelania hupensis
- Aquaria maintenance
- Growth of immature B. glabrata
- Snail egg clutch collection
- Growth conditions until patency
- Control of rotifers and other commensals living in snail aquaria
- Gel snail food preparation
- Preparation of mud base medium for growth of Nostoc (cyanobacteria)
Procedures common to maintaining Schistosoma spp. life cycles
- Percutaneous exposure of mice to Schistosoma mansoni
- Perfusion of adult worms from infected mice
- Behavior of Schistosoma mansoni cercariae
- Preparation of in vitro schistosomules
- Collection of lung Schistosoma mansoni schistosomules
- Culturing schistosomula
- Isolating Schistosoma spp. eggs from murine liver and gut
- Microscopic evaluation of sporocyst development in live Biomphalaria glabrata
- Shedding cercariae from patent snails
- Exposure of snails to miracidia
- Positivity shedding
- Quantitating Schistosoma spp. cercariae
Procedures common to S. japonicum in the Oncomelania spp. snail host
Procedures common to S. haematobium in the Bulinus spp. snail host
Molecular and cell culture products
- Biomphalaria glabrata embryonic cell (Bge) cell culture
- Genomic DNA extraction from schistosome intermediate hosts and parasite stages
- Non-invasive DNA transfection to B. glabrata using polyethylenimine (PEI)
- Preparation of soluble egg antigen (SEA) S. japonicum eggs
- RNA extraction from schistosome intermediate hosts and parasite stages using RNAzol ®
