Protocols
SRC protocols are continuously refined using the latest data and shared with the schistosomiasis community. These protocols are currently utilized for SRC operations and are divided into 6 main categories.
Procedures common to maintaining intermediate snail hosts of Schistosoma spp. Procedures common to maintaining Schistosoma spp. life cycles Procedures common to S. japonicum in the Oncomelania spp. snail host Procedures common to S. haematobium in the Bulinus spp. snail host Biomphalaria glabrata embryonic cell line Molecular and cell culture products
Procedures common to maintaining intermediate snail hosts of Schistosoma spp.
- Artificial pond water for the cultivation of Biomphalaria glabrata, Bulinus truncatus and Oncomelania hupensis
- Aquaria maintenance for snails
- Growth of immature Biomphalaria glabrata
- Snail egg clutch collection
- B. glabrata growth conditions following exposure to Schistosoma
- Control of rotifers and other commensals living in snail aquaria
- Gel snail food preparation
- Preparation of mud base medium for growth of Nostoc
- Fixing Biomphalaria glabrata snails for dissection
Procedures common to maintaining Schistosoma spp. life cycles
- Percutaneous exposure of mice to Schistosoma mansoni
- Perfusion of adult worms from infected mice
- Behavior of Schistosoma mansoni cercariae
- Preparation of in vitro schistosomules
- Collection of lung Schistosoma mansoni schistosomules
- Culturing schistosomula
- Isolating Schistosoma spp. eggs from murine liver and gut
- Microscopic evaluation of sporocyst development in live Biomphalaria glabrata
- Shedding cercariae from patent snails
- Exposure of snails to miracidia
- Positivity shedding
- Quantitating Schistosoma spp. cercariae
Procedures common to S. japonicum in the Oncomelania spp. snail host
Procedures common to S. haematobium in the Bulinus spp. snail host
Biomphalaria glabrata embryonic cell line
Molecular and cell culture products
Inquire About Free Reagents
The SRC offers infected snails, parasite eggs, the Bge molluscan cell line and other materials free of charge. Recipients pay for shipping and handling.