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# Biomedical Research Institute

Schistosomiasis Resource Center. Biorepository Services

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- [Homepage](https://www.afbr-bri.org/) - Nonprofit advancing global health for 40+ years through biorepository services and NIH-supported schistosomiasis research and reagent supply.
- [Biorepository Services](https://www.afbr-bri.org/biorepository-specimen-storage/) - Biomedical Research Institute The Biomedical Research Institute provides excellent biorepository service for your valuable biospecimens.Turn-Key Biorepository SolutionsWe get it! BRI is dedicated to protecting your most valuable assets. We understand how storage and access to your precious materials are critical to your research and product or drug development. Request a FREE QUOTEWe care! BRI has
- [Turn-Key Biorepository Solutions](https://www.afbr-bri.org/biorepository-specimen-storage/turn-key-biobanking-solutions/) - Biomedical Research Institute Turn-Key Biorepository SolutionsOur team welcomes the opportunity to meet your individual biobanking needs.Please email your contact information and one of our experts will connect with you shortly: biobank@afbr-bri.org Request a FREE QUOTEFor more information about obtaining specimens from the ACTG and IMPAACT clinical trials networks, go to specimen repository.org Secure, Cost-effective and
- [Contact BRI](https://www.afbr-bri.org/contact-bri/) - Contact BRI 9410 Key West Rockville, MD 20850 Phone: 301-329-0412 Contact Us "*" indicates required fields CommentsThis field is for validation purposes and should be left unchanged.Name* First Last Email* PhoneChoose a Department* Biorepository Services Schistosomiasis Resource Center Message*
- [Careers](https://www.afbr-bri.org/careers/) - Careers There are no positions currently available. Please check back later or submit your resume for possible upcoming openings. Submit Your ResumeLocation: 9410 Key West Avenue, Rockville, Maryland USA 20850 Visit www.afbr-bri.org
- [S. haematobium cercariae collection from Bulinus truncatus](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/s-haematobium-cercariae-collection-from-bulinus-truncatus/) - Schistosomiasis Resource CenterStandard Operating ProceduresS. haematobium cercariae collection from Bulinus truncatus S. haematobium cercariae collection from Bulinus truncatusDownload PDFMATERIALS Proper PPE: disposable gloves and lab coat (face shield may also be necessary). Patent B.truncatus are cercariae positive and at biosafety level 2 status (BSL-2). Cercariae can penetrate exposed skin and therefore, protocols for handling positive
- [SRC Reagents](https://www.afbr-bri.org/schistosomiasis/schistosomiasis-resource-center-reagents/) - Schistosomiasis Resource CenterSRC Reagents Biomedical Research Institute Schistosomiasis Resource Center ReagentsThe Schistosomiasis Resource Center was established by the Biomedical Research Institute, Rockville, Maryland in 1995. For a comprehensive list of schistosomiasis-related reagents available from the SRC, write to Margaret Mentink-Kane, Ph.D. CONTACT USSNAILS Unexposed (naïve) Biomphalaria glabrata (NMRI, M-line and BS-90 strains) Unexposed (naïve) Bulinus truncatus
- [Contact SRC](https://www.afbr-bri.org/schistosomiasis/contacts/) - Schistosomiasis Resource CenterContact SRC 9410 Key West Rockville, MD 20850 Principal Investigator Margaret Mentink-Kane, PhD Schistosomiasis Resource Center Biomedical Research Institute Rockville, Maryland Phone 301-329-0412 Email: mmentinkkane@afbr-bri.org Laboratory Operations Coordinator Gabrielle Bate Schistosomiasis Resource Center Biomedical Research Institute Rockville, Maryland Phone: 301-329-0215 Email: gbate@afbr-bri.org 5601 Fishers Lane Rockville, MD 20852 Program Officer Dierdre Joy, PhD
- [Aquaria Maintenance](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/aquaria-maintenance/) - Schistosomiasis Resource CenterStandard Operating ProceduresAquaria Maintenance Aquaria maintenanceChanging containers of snails (aquaria maintenance)Authors: Fred Lewis, PhD and Frances BarnesDownload PDF file Introduction Biomphalaria, Bulinus, and Oncomelania can be maintained in a wide variety of containers. For practical reasons, we prefer to use shallow trays, rather than deep aquaria for maintenance. Whatever the container or aquarium
- [Exposure of snails to miracidia](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/exposure-of-snails-to-miracidia/) - Schistosomiasis Resource CenterStandard Operating ProceduresExposure of snails to miracidia Exposure of snails to miracidiaAuthors: Fred Lewis, PhD, Sarah Schmid, MScDownload PDF file Introduction Schistosome eggs hatch readily when placed in water, depending upon the tissues from which they are obtained. Concentrated egg pellets, when diluted in copious amounts of water, can yield large numbers of
- [Standard Operating Procedures](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/) - Schistosomiasis Resource CenterStandard Operating Procedures SCHISTOSOMIASIS RESOURCE CENTER STANDARD OPERATING PROCEDURESThese Standard Operating Procedures are used in the Schistosomiasis Resource Center to maintain the life cycles of Schistosoma spp. The SOPs are divided into five categories. Image of S.mansoni schistosomula cultured in vitro Procedures common to maintaining intermediate snail hosts of Schistosoma spp. Artificial pond
- [Artificial Pond Water](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/artificial-pond-water/) - Schistosomiasis Resource CenterStandard Operating ProceduresArtificial Pond Water Artificial pond waterFor the cultivation of Biomphalaria glabrata, Bulinus truncatus and Oncomelania hupensis in the laboratory settingDownload PDF file The Schistosomiasis Resource Center (SRC) uses an ion exchange system to purify the water supplied to the laboratory. Use purified water to prepare the 4 stock solutions below: FeCl3
- [Leadership](https://www.afbr-bri.org/leadership/) - Leadership BRI Board of DirectorsPhilip LoVerde, Ph.D. PresidentProfessor, Department of Biochemistry, University of Texas Health Science CenterJohn B. Sacci, Ph.D. Vice PresidentAssociate Professor, Department of Microbiology & Immunology, University of Maryland School of MedicineBob Burleson, MBA TreasurerChief Executive Officer, IOMAXISBrian GainesDirectorChief Executive Officer, Learning UndefeatedChristopher L. White, Esq. DirectorGeneral Counsel, Chief Policy Officer & Secretary
- [Schistosomiasis Resource Center](https://www.afbr-bri.org/schistosomiasis/) - Schistosomiasis Resource Center Biomedical Research Institute The Schistosomiasis Resource Center at the Biomedical Research Institute is a unique schistosomiasis research supply laboratory funded by the NIAID (NIAID-NIH)The SRC provides Schistosoma life cycle reagents free of charge to scientists, students, biotech and independent investigators in the U.S. and around the world. ORDER REAGENTSArtwork by Myla Leung
- [Growth conditions until patency](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/growth-conditions-until-patency/) - Schistosomiasis Resource CenterStandard Operating ProceduresGrowth conditions until patency B. glabrata growth conditions until patencyAuthor Fred Lewis, PhD and André Miller Download PDF file Introduction The length of the prepatent phase of S. mansoni in B. glabrata is highly dependent on maintenance temperature. If the snails are maintained continually at 26ºC, some snails can begin to
- [Shedding cercariae from patent snails](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/shedding-cercariae-from-patent-snails/) - Schistosomiasis Resource CenterStandard Operating ProceduresShedding cercariae from patent snails Shedding cercariae from patent snailsAuthors Fred Lewis and Frances BarnesIntroduction After cercariae mature within the snail, they are then liberated (shed) into the surrounding water. In field conditions, cercariae typically emerge in greatest numbers in the daytime. In the laboratory and for experimental purposes, investigators can
- [Brian Gaines](https://www.afbr-bri.org/leadership/brian-gaines/) - LeadershipBrian Gaines Brian GainesDIRECTORChief Executive Officer, Learning UndefeatedBrian Gaines is a dynamic business and social entrepreneur, certified executive and leadership coach with over 25 years of experience driving growth and innovation across multiple sectors. Since 2012, he has served as the Chief Executive Officer of Learning Undefeated, an award-winning national nonprofit organization dedicated to advancing
- [Biomphalaria glabrata embryonic cell (Bge) culture](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/biomphalaria-glabrata-embryonic-cell-bge-culture/) - Schistosomiasis Resource CenterStandard Operating ProceduresBiomphalaria glabrata embryonic cell (Bge) culture Biomphalaria glabrata embryonic cell (Bge) cultureAuthors: Wannaporn Ittiprasert, PhD André MillerDownload PDF file Shipped Bge cells: high density cells are shipped in a 50mL cell culture flask filled with Bge medium to prevent excessive movement during shipping. Allow cells to settle overnight at 26oC. •
- [Collection of S. haematobium eggs from hamsters](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/collection-of-s-haematobium-eggs-from-hamsters/) - Schistosomiasis Resource CenterStandard Operating ProceduresCollection of S. haematobium eggs from hamsters Collection of S. haematobium eggs from hamstersAuthors Yung-san Liang, Mei-Shei Su and Fred LewisIntroduction The procedure for collection of S. haematobium eggs from the tissues of hamsters is similar to that described for the collection of S. mansoni eggs from mice. In practice, however, the majority of recoverable S.
- [Perfusion of adult S. haematobium worms from infected hamsters](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/perfusion-of-s-haematobium-from-hamsters/) - Schistosomiasis Resource CenterStandard Operating ProceduresPerfusion of adult S. haematobium worms from infected hamsters Perfusion of adult S. haematobium worms from infected hamstersCollection of S. haematobium adult worms is much the same principle as that described for collection of adult S. mansoni worms from mice (e.g., perfusion from the portal venous system). The best time for recovery of adult worms
- [Exposure of LVG Golden Syrian Hamsters to Schistosoma haematobium cercariae](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/exposure-of-hamsters-to-schistosoma-haematobium-cercariae/) - Schistosomiasis Resource CenterStandard Operating ProceduresExposure of LVG Golden Syrian Hamsters to Schistosoma haematobium cercariae Exposure of LVG Golden Syrian Hamsters to Schistosoma haematobium cercariaeAuthors: Yung-san Liang, PhD , Mei-Shei Su and Fred Lewis, PhDDownload PDF file Introduction The abdomen is the preferred site for percutaneous exposure with S. haematobium in hamsters Materials Cercarial shed from
- [Maintenance of Bulinus truncatus truncatus](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/maintenance-of-bulinus-truncatus-truncatus-snails/) - Schistosomiasis Resource CenterStandard Operating ProceduresMaintenance of Bulinus truncatus truncatus Maintenance of Bulinus truncatus truncatusAuthors Yung-san Liang, Mei-Shei Su, Laksiri Karunaratne, and Fred LewisOverview and recommendations For the most part, Bulinus truncatus truncatus snails grow well under the same conditions as those described for Biomphalaria spp. snails. There are a few differences which are important to consider when working with B.
- [FAQs](https://www.afbr-bri.org/schistosomiasis/faq/) - Schistosomiasis Resource CenterFAQs SCHISTOSOMIASIS RESOURCE CENTER FAQsThe Schistosomiasis Resource Center at the Biomedical Research Institute (BRI) is a unique schistosomiasis research supply laboratory funded by the NIAID at the National Institutes of Health. Who can request the cost-free reagents from the Schistosomiasis Resource Center? Any academic, government, or commercial laboratory that is involved in schistosomiasis
- [Edwin Ashourian](https://www.afbr-bri.org/leadership/edwin-ashourian/) - LeadershipEdwin Ashourian Edwin Ashourian, MBAEXECUTIVE DIRECTOR, CHIEF FINANCIAL OFFICERInstitutional OfficialEdwin Ashourian is Executive Director, Chief Financial Officer for Biomedical Research Institute, where he works with the executive leadership on financial strategy, business leadership, strategic planning and leads all aspects of financial management functions including corporate finance, accounting, audit, treasury, and human resources activities. He is
- [Margaret Mentink-Kane](https://www.afbr-bri.org/leadership/margaret-mentink-kane/) - LeadershipMargaret Mentink-Kane Margaret Mentink-Kane, Ph.D.EXECUTIVE DIRECTOR, CHIEF SCIENTIFIC OFFICERPrincipal Investigator NIH-NIAID Schistosomiasis Resource CenterMargaret Mentink-Kane, Ph.D. has managed the Schistosomiasis Resource Center (SRC) since January 2015. After receiving a B.S. in Biology from the University of Wisconsin-Stevens Point, she completed her Ph.D. in Immunology and Microbiology at Temple University, Philadelphia. Following a post-doctoral fellowship with
- [Philip LoVerde](https://www.afbr-bri.org/leadership/philip-loverde/) - LeadershipPhilip LoVerde Philip LoVerde, Ph.D.PRESIDENTProfessor, Department of Biochemistry, University of Texas Health Science CenterDr. Philip T. LoVerde is a Professor at the University of Texas Health Science Center in San Antonio, Texas. His research interests are in host-parasite interactions, especially those that involve the human blood fluke, Schistosoma. His current research involves vaccine development, role
- [Ordering](https://www.afbr-bri.org/schistosomiasis/ordering/) - Schistosomiasis Resource CenterOrdering Biomedical Research Institute Schistosomiasis Resource Center The SRC offers infected mammals, intermediate hosts, Bge cells, molecular reagents and other materials free of charge. Distribution of schistosomes and their intermediate hosts is from the Biomedical Research Institute in Rockville, Maryland. Molecular reagents are distributed directly from BEI Resources in Manassas, Virginia. Available life
- [Snail Egg clutch collection](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/snail-egg-clutch-collection/) - Schistosomiasis Resource CenterStandard Operating ProceduresSnail Egg clutch collection Snail Egg clutch collectionAuthor Fred Lewis, PhDDownload PDF file Introduction The egg clutches (masses) of Biomphalaria glabrata and Bulinus truncatus truncatus typically contain a variable number of embryos. From the time the egg clutches are deposited by the adult snails, embryos begin to emerge in about 2
- [Shedding Oncomelania for S.japonicum cercariae](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/shedding-oncomelania-for-s-japonicum-cercariae/) - Schistosomiasis Resource CenterStandard Operating ProceduresShedding Oncomelania for S.japonicum cercariae Schistosoma japonicum cercariae collection from patent Oncomelania sppAuthors: Laksiri Karunaratne, MS, Fred Lewis PhD, Sarah Li, Yung-san Liang, PhD and Mei-Shei SuDownload PDF file Equipment Dissecting microscope Materials and Reagents Petri dishes Pasteur pipettes Artificial Pond Water (water) Fine forceps or small hypodermic needle (e.g., 25
- [RNA extraction from schistosome intermediate hosts and parasite stages using RNAzol®](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/rna-extraction-from-schistosome-intermediate-hosts-and-parasite-stages-using-rnazol/) - Schistosomiasis Resource CenterStandard Operating ProceduresRNA extraction from schistosome intermediate hosts and parasite stages using RNAzol® RNA extraction from schistosome intermediate hosts and parasite stages using RNAzol®Authors: Wannaporn Ittiprasert and Andre Miller RNA extraction from schistosome intermediate hosts and parasite stages using RNAzol® RT mRNA isolation This protocol yields two RNA fractions; mRNA-containing fraction > 200
- [Quantitating Schistosoma spp. cercariae](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/quantitating-schistosoma-spp-cercariae/) - Schistosomiasis Resource CenterStandard Operating ProceduresQuantitating Schistosoma spp. cercariae Quantitating Schistosoma spp. cercariaeAuthor: Fred Lewis, PhDDownload PDF file Introduction Numerous procedures in a schistosomiasis laboratory necessitate counting cercariae. One such procedure is the exposure of small mammals, such as mice, to cercariae for infection, where it is important to obtain an accurate estimate of the number
- [Preparation of soluble egg antigen (SEA) S. japonicum eggs](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/preparation-of-soluble-egg-antigen-sea-s-japonicum-eggs/) - Schistosomiasis Resource CenterStandard Operating ProceduresPreparation of soluble egg antigen (SEA) S. japonicum eggs Preparation of soluble egg antigen (SEA) S. japonicum eggsAuthor: Fred Lewis Introduction One of the most widely used antigenic preparations from schistosomes is SEA (soluble egg antigen). The crude extract obtained from the mature eggs is isolated from the tissues of the
- [Preparation of mud base medium for growth of Nostoc](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/preparation-of-mud-base-medium-for-growth-of-nostoc/) - Schistosomiasis Resource CenterStandard Operating ProceduresPreparation of mud base medium for growth of Nostoc Preparation of mud base medium for growth of NostocAuthors: Yung-san Liang, Mei-Shei Su, Mitzi Sereno and Frances BarnesDownload PDF file – need PDF Introduction For growth in the laboratory, especially of the fastidious Oncomelania snails, the cyanobacterium Nostoc sp. serves as a
- [Preparation of in vitro schistosomules](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/preparation-of-in-vitro-schistosomules/) - Schistosomiasis Resource CenterStandard Operating ProceduresPreparation of in vitro schistosomules Preparation of in vitro schistosomulesAuthors: Fred Lewis, PhD and André MillerDownload PDF file Introduction A number of methods are used to transform cercariae into the next developmental stage, the schistosomule. Two of the more commonly used laboratory methods are presented here. Currently the Schistosomiasis Resource Center
- [Positivity shedding](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/positivity-shedding/) - Schistosomiasis Resource CenterStandard Operating ProceduresPositivity shedding Determination of patency via shedding in Biomphalaria glabrata exposed to S. mansoniAuthor: Legendre Miller, Jr. and Sarah Schmid, MSc.Download PDF file Introduction For B. glabrata exposed to S. mansoni miracidia, wait 4 weeks post-infection before testing for the presence of cercariae. Testing snails individually is key to establishing a
- [Perfusion of adult worms from mice](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/perfusion-of-adult-worms-from-mice/) - Schistosomiasis Resource CenterStandard Operating ProceduresPerfusion of adult worms from mice Perfusion of adult worms from miceAuthors: Fred Lewis, PhD , Sarah Schmid, MScDownload PDF file Introduction One of the more common procedures in a schistosome life cycle laboratory is the collection of adult worms from the definitive mammalian host. In a bisexual worm infection in
- [Genomic DNA extraction from schistosome intermediate hosts and parasite stages](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/genomic-dna-extraction-from-schistosome-intermediate-hosts-and-parasite-stages/) - Schistosomiasis Resource CenterStandard Operating ProceduresGenomic DNA extraction from schistosome intermediate hosts and parasite stages Genomic DNA extraction from schistosome intermediate hosts and parasite stagesAuthors: Wannaporn Ittiprasert, PhD and André MillerDownload PDF file DNAzol® GENOMIC DNA ISOLATION REAGENT Using this reagent, the isolated DNA can be used without additional purification for Southern analysis, dot blot hybridization,
- [Gel snail food preparation](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/gel-snail-food-preparation/) - Schistosomiasis Resource CenterStandard Operating ProceduresGel snail food preparation Snail gelAuthor: Fred Lewis, PhDDownload PDF file Introduction Snail gel is an ideal food supplement to the lettuce and algae (Nostoc spp.) that is normally fed to laboratory snail colonies. Currently, the NIH NIAID Schistosomiasis Resource Center uses snail gel as a supplement to feed neonates, juveniles
- [Culturing schistosomula](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/culturing-schistosomula/) - Schistosomiasis Resource CenterStandard Operating ProceduresCulturing schistosomula Culturing schistosomulaAuthors: Tori C. Freitas and Edward J. PearceDownload PDF file Introduction Infectious cercariae are transformed in vitro into schistosomula. Schistosomula can be cultured in vitro in a complex medium. These in vitro cultured schistosomula do not grow at the same rate as those in a permissive host, nor
- [Percutaneous exposure of mice to Schistosoma mansoni](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/percutaneous-exposure-of-mice-to-schistosoma-mansoni/) - Schistosomiasis Resource CenterStandard Operating ProceduresPercutaneous exposure of mice to Schistosoma mansoni Percutaneous exposure of mice to Schistosoma mansoniAuthor: Fred Lewis, PhDDownload PDF file – need PDF Introduction Percutaneous exposure of mice to S. mansonivia the tail offers significant advantages over other means of infection. Skin penetration is the natural route of cercarial infection, andthere is
- [Non-invasive DNA transfection to B. glabrata using polyethylenimine (PEI)](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/non-invasive-dna-transfection-to-b-glabrata-using-polyethylenimine-pei/) - Schistosomiasis Resource CenterStandard Operating ProceduresNon-invasive DNA transfection to B. glabrata using polyethylenimine (PEI) Non-invasive DNA transfection of B. glabrata using polyethylenimine (PEI)Download PDF file Chemicals – Ampicillin (Amp) – 0.2 mg/ml of plasmid DNA (pDNA) – PEI transfection reagent – Sterile distilled water Protocols Day 1: Treat snail(s) with 100 mg/ml Amp in distilled water
- [Molecular technique for sexing cercariae](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/molecular-technique-for-sexing-cercariae/) - Schistosomiasis Resource CenterStandard Operating ProceduresMolecular technique for sexing cercariae Molecular technique for sexing cercariaeAuthors: Tori C. Freitas, Sarah Galanti, and Edward J. Pearce Introduction Snails infected with a single miracidium will release a clonal population of cercariae upon becoming patent. Because the sex of the parasite is determined at the egg stage, all cercariae shedding
- [Microscopic evaluation of sporocyst development in live Biomphalaria glabrata](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/microscopic-evaluation-of-sporocyst-development-in-live-biomphalaria-glabrata/) - Schistosomiasis Resource CenterStandard Operating ProceduresMicroscopic evaluation of sporocyst development in live Biomphalaria glabrata Microscopic evaluation of sporocyst development in live Biomphalaria glabrataAuthors: Fred Lewis, PhDIntroduction The successive intramolluscan stages of schistosome development are called primary (mother) and secondary (daughter) sporocysts. The black mantle pigmentation in field-collected B.glabrata makes intramolluscan stages difficult to detect using a dissecting
- [Isolating Schistosoma spp. eggs from murine liver and gut](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/isolating-schistosoma-spp-eggs-from-murine-liver-and-gut/) - Schistosomiasis Resource CenterStandard Operating ProceduresIsolating Schistosoma spp. eggs from murine liver and gut Isolating Schistosoma spp. eggs from murine liver and gutAuthors: Fred Lewis, PhD, Frances Barnes, Mei-Shei Su and Yung-san Liang, PhDDownload PDF file Introduction Schistosome eggs are collected for experimental use primarily from either livers or intestinal tissue of infected animal hosts. This
- [Growth of immature Biomphalaria glabrata](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/growth-of-immature-biomphalaria-glabrata/) - Schistosomiasis Resource CenterStandard Operating ProceduresGrowth of immature Biomphalaria glabrata Growth of immature Biomphalaria glabrataAuthor Fred Lewis, PhDDownload PDF file Introduction The growth rate of B. glabrata is highly dependent on the ambient temperature at which the snails are maintained. Newly-hatched B. glabrata can grow to about 5 mm (shell diameter) in 3-4 weeks (or sooner)
- [Control of rotifers and other commensals living in snail aquaria](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/control-of-rotifers-and-other-commensals-living-in-snail-aquaria/) - Schistosomiasis Resource CenterStandard Operating ProceduresControl of rotifers and other commensals living in snail aquaria Control of rotifers and other commensals found in Biomphalaria glabrata and other snail coloniesAuthors: Fred Lewis, PhD and Yung-san Liang, PhDDownload PDF file Introduction There are several invertebrates that can interfere with the growth of Biomphalaria glabrata and/or the development and
- [Collection of lung Schistosoma mansoni schistosomules](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/collection-of-lung-schistosoma-mansoni-schistosomules/) - Schistosomiasis Resource CenterStandard Operating ProceduresCollection of lung Schistosoma mansoni schistosomules Collection of lung Schistosoma mansoni schistosomulesAuthors: Fred Lewis, PhDIntroduction Schistosoma mansoni lung schistosomules can be obtained in greatest numbers from mice ~5-8 days after cercarial penetration of the skin. The lung tissue must be chopped finely enough to allow the schistosomules to migrate out of the
- [Collecting Schistosoma japonicum eggs from mouse tissues](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/collecting-schistosoma-japonicum-eggs-from-mouse-tissues/) - Schistosomiasis Resource CenterStandard Operating ProceduresCollecting Schistosoma japonicum eggs from mouse tissues Collecting Schistosoma japonicum eggs from mouse tissuesAuthors Yung-san Liang, Mei-Shei Su, and Laksiri KarunaratneIntroduction The procedure for collection of S. japonicum eggs from mouse tissues is essentially the same as that used for collecting S. mansoni eggs from mouse tissues. Recoverable S. japonicum eggs will be found in the liver
- [Biomphalaria glabrata embryonic cell (Bge) cell culture](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/biomphalaria-glabrata-embryonic-cell-bge-cell-culture/) - Schistosomiasis Resource CenterStandard Operating ProceduresBiomphalaria glabrata embryonic cell (Bge) cell culture Biomphalaria glabrata embryonic cell (Bge) culture protocolAuthors: Wannaporn Ittiprasert, PhD , André MillerDownload PDF file Materials Bge Medium 1 liter: Schneider’s Drosophila Medium 220 mL lactalbumin hydrolysate 4.5 g Galactose 1.3g MilliQ H2O up to 900mL Gentamycin (10 mg/mL stock) 2.0 mL Phenol Red
- [Behavior of Schistosoma mansoni cercariae](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/behavior-of-schistosoma-mansoni-cercariae/) - Schistosomiasis Resource CenterStandard Operating ProceduresBehavior of Schistosoma mansoni cercariae Behavior of Schistosoma mansoni cercariaeAuthor: FredLewis, PhDDownload PDF file Introduction The cercariae of most trematode species exhibit very specific characteristic behavior patterns. Learning to distinguish normal from abnormal cercarial behavior is very important, since abnormal cercarial behavior likely indicates problem areas that might adversely affect the
- [A histochemical method for identifying sex of Schistosoma mansoni cercariae (under construction)](https://www.afbr-bri.org/schistosomiasis/standard-operating-procedures/a-histochemical-method-for-identifying-sex-of-schistosoma-mansoni-cercariae-under-construction/) - Schistosomiasis Resource CenterStandard Operating ProceduresA histochemical method for identifying sex of Schistosoma mansoni cercariae (under construction) A histochemical method for identifying sex of Schistosoma mansoni cercariae (under construction)Author: James Liberatos Introduction The sex of Schistosoma mansoni cercariae can be determined rapidly and reliably using a modified C-banding technique, originally developed for use in chromosome banding. S. mansoni females
- [Resources](https://www.afbr-bri.org/schistosomiasis/resources/) - Schistosomiasis Resource CenterResources SCHISTOSOMIASIS RESOURCE CENTER RESOURCESThe Schistosomiasis Resource Center at the Biomedical Research Institute (BRI) is a unique schistosomiasis research supply laboratory funded by the NIAID at the National Institutes of Health. Links The American Society of Tropical Medicine and Hygiene The American Society of Parasitologists BEI Resources Biomedical Research Institute Biomphalaria genome initiative CEBio S.
- [Biorepository Thank You](https://www.afbr-bri.org/biorepository-thank-you/) - Your Biorepository Service Provider Does Make a DifferenceWe get it! BRI is dedicated to protecting your most valuable assets. We understand how storage and access to your precious materials are critical to your research and product or drug development. We care! BRI has been a trusted biorepository resource to the life sciences community for nearly
- [Rachael Widener](https://www.afbr-bri.org/leadership/rachael-widener/) - LeadershipRachael Widener Rachael Widener, Ph.D.DIRECTORAlliance Manager, Business Development & Licensing Boehringer Ingelheim Animal HealthRachael Widener is currently an Alliance Manager in the Business Development & Licensing division of Boehringer Ingelheim Animal Health (BIAH). In her role, she identifies, develops and nurtures long-term value-creating relationships designed to enable and/or accelerate the achievement of strategic objectives of
- [Christopher White](https://www.afbr-bri.org/leadership/christopher-white/) - LeadershipChristopher White Christopher L. White, Esq.DIRECTORGeneral Counsel, Chief Policy Officer & Secretary of the Advanced Medical Technology Association (AdvaMed) in Washington, D.C.Mr. Christopher White serves as the Association’s Chief Legal Officer and AdvaMed’s Chief Policy Officer, leading the Association’s work to develop and promote policy positions enhancing patient access to medical technology world wide. Mr.
- [John Sacci](https://www.afbr-bri.org/leadership/john-sacci/) - LeadershipJohn Sacci John B. Sacci, Ph.D.VICE PRESIDENTAssociate Professor, Department of Microbiology & Immunology, University of Maryland School of MedicineDr. John B. Sacci received his PhD from the University of Maryland, Baltimore. He then did post-doctoral fellowships at the Johns Hopkins School of Public Health and the University of Maryland School of Medicine. His research as
- [Bob Burleson](https://www.afbr-bri.org/leadership/bob-burleson/) - LeadershipBob Burleson Bob Burleson, MBATREASURERChief Executive Officer, IOMAXISBob Burleson has over thirty years of accounting, financial and operational experience encompassing active involvement in major strategic decisions. His background also includes Acquisitions and Divestitures with proven leadership ability to manage the revenue generating employees of a company. He is highly skilled in strategic and financial planning
- [About](https://www.afbr-bri.org/about/) - Biomedical Research Institute Schistosomiasis Resource CenterThe Schistosomiasis Resource Center (SRC) at BRI is a National Institutes of Health- supported production and supply laboratory that provides schistosomiasis research reagents free of charge to scientists and students throughout the U.S. and the international research community. Schistosomiasis is an infectious disease caused by a parasitic worm with a life cycle

## Events

- [Schistosoma spp. Life Cycle Training Course April 2026](https://www.afbr-bri.org/event/schistosoma-spp-life-cycle-training-course/) - Please contact Margaret Mentink-Kane for questions about the Course. Email: mmentinkkane@afbr-bri.org Phone: 301-329-0412 April 1-3, 2026 The Schistosomiasis Resource Center at the Biomedical Research Institute offers a training course on the maintenance of three Schistosoma spp. life cycles: S. haematobium, S. japonicum and S.mansoni. The course is designed to acquaint researchers working in the field of schistosomiasis with protocols for maintaining the complete parasite life cycle in their own laboratory. The course provides both lectures and wet-lab exercises, including...
- [Conference Room Dedication for W. Patrick Monaghan, PhD](https://www.afbr-bri.org/event/conference-room-dedication-for-w-patrick-monaghan-phd/) - A dedication ceremony in honor of W. Patrick Monaghan, PhD, took place on December 12, 2023, at the Biomedical Research Institute (BRI) following the quarterly meeting of BRI’s Board of Directors.
- [17º International Symposium on Schistosomiasis](https://www.afbr-bri.org/event/17o-international-symposium-on-schistosomiasis/) - 17º International Symposium on Schistosomiasis November 2024 Salvador, Brazil Event Details and Online Registration Please contact: Thiago Almeida Pereira, MS, PhD Office#: 1 (650) 724-9116 Mobile#: 1 (202) 813-6153 Email: thiagoap@stanford.edu

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## Tags

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## Event Categories

- [Schistosomiasis](https://www.afbr-bri.org/events/category/schistosomiasis/)