2016 Life Cycle and Molecular Training Course Participants

Schistosoma spp. Life Cycle Training Course

The NIH-NIAID Schistosomiasis Resource Center offers a semiannual training course on the maintenance of Schistosoma life cycles: S. haematobium, S. japonicum and S.mansoni. The course is designed to acquaint investigators working in the field of schistosomiasis with protocols for maintaining the complete parasite life cycle in their own laboratory and provides both wet lab and lectures. Protocols include: snail cultivation, exposure of snails to miracidia, shedding patent snails for cercariae collection, mice and hamster infections and collection of adult worms and eggs from tissues. The 2 ½ day course also provides a presentation describing NIH grants and research resources by Glen McGugan, PhD, from the Parasitology and International Programs Branch, NIAID.

We anticipate that attendees will come away with a greater appreciation of the basic biology of Schistosoma spp. parasites and the ability to maintain the life cycle in their lab.

This is a great learning opportunity for researchers of all levels who work with schistosomes, snails and rodent models of infection.

Interested in attending the Schistosoma spp. Life Cycle Training Course?
Contact Margaret Mentink-Kane, PhD at mmentinkkane@afbr-bri.com

There is no fee associated with the course but attendees are responsible for their own travel, lodging, and meals. We are located at 9410 Key West Avenue, Rockville MD 20850. Phone 301-881-3300

Schistosomiasis Molecular Training Course

The next course is scheduled for December 6-8, 2017 

“Gene Editing of Adult Schistosomes by CRISPR/cas”

The Schistosomiasis Resource Center Molecular Training Course, “Gene Editing of Adult Schistosomes by CRISPR/Cas” will be held December 6-8, 2017 at BRI. Attendees will receive hands-on training involving CRISPR/Cas-based knock down of target gene expression in adult worms in vitro. Lectures on primer design and data analysis will be given by SRC staff, with additional lectures on recent research advances from visiting scientists (to be announced). Specific techniques for the hands-on training will include:

  • Adult schistosome culture
  • Total RNA and DNA isolations
  • cDNA preparation and quantitative PCR
  • Genomic cleavage assays and gel electrophoresis

This is a great learning opportunity for researchers of all levels who want to apply molecular techniques to their studies of schistosomes and snails. Anyone interested in attending the Molecular Training Course can contact  Margaret Mentink-KaneThere is no fee associated with the course, but attendees are responsible for their own travel, lodging, and meals.